pH-responsive characteristics of chitosan-based blends for controlling the adhesivity of cells
| Autor: | Yi-Chen Ethan Li, Tai-Horng Young, Nai-Chen Cheng, Hsu-Hsien Chang, Jyh-Horng Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
Cell macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Polyvinyl alcohol Chitosan chemistry.chemical_compound Tissue engineering medicine biology Chemistry technology industry and agriculture General Chemistry equipment and supplies 021001 nanoscience & nanotechnology Control cell 0104 chemical sciences Fibronectin medicine.anatomical_structure Cell culture Polycaprolactone biology.protein Biophysics 0210 nano-technology |
| Zdroj: | Journal of the Taiwan Institute of Chemical Engineers. 111:34-43 |
| ISSN: | 1876-1070 |
| DOI: | 10.1016/j.jtice.2020.04.022 |
| Popis: | Cell detachment is a routine process to recover seeded cells from culture systems. However, traditional enzymatic methods might easily induce senescence or apoptosis of cells during the routine passage process due to an improper handing process. The pH-responsive characteristic of chitosan has been demonstrated to control cell attachment/detachment by precise adjustment of medium pH within 1 h without additional enzymatic treatment and extensive washing steps. But the time 1 h is not satisfying to all applications to recover adherent cells from chitosan surface. The purpose of this study is to regulate pH-responsive characteristics of chitosan by blending with non-pH-responsive biomaterials, polycaprolactone (PCL) and polyvinyl alcohol (PVA) to provide a gentle rapid method for cell detachment. In this study, PCL could delay the pH-responsive characteristics of chitosan to reduce cell detachment ratio ( 95%) from the chitosan/PVA blended surface within 30 min after raising medium pH to 7.65. We further discuss the related mechanism among cell detachment, fibronectin, and chitosan-based blends. These results indicate non-pH-responsive PCL and PVA could be added into pH-responsive chitosan to develop a rapid non-enzymatic strategy to control cell detachment rate for cell passage and various tissue engineering applications. |
| Databáze: | OpenAIRE |
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