Application of luminescence spectral assay to evaluate the results of a scientific experiment

Autor: A. V. Krasnikov, S. V. Akchurin, S. V. Larionov, I. V. Akchurina, E. S. Krasnikova
Rok vydání: 2020
Předmět:
Zdroj: Journal of Physics: Conference Series. 1679:022019
ISSN: 1742-6596
1742-6588
DOI: 10.1088/1742-6596/1679/2/022019
Popis: The method of characterization of functional state of epithelium of mucous membrane of alveolar glands of poultry proventriculus, using luminescence spectral assay with fluorescent dyes Ethidium bromide (EtBr) and 5-([4.6-Dichlorotriazin-2-yl]amino) fluorescein hydrochloride (DTAF) is offered. The dynamics of nucleic acids and proteins content in chickens’ cells under experimental klebsiellosis in antibiotic-treated poultry is established using this method. It is found that at the 2th – 7th days of treatment the markers of the cells functional status in the experimental group chickens are similar to those of poultry of the intact group.
Databáze: OpenAIRE