| Autor: |
Csaba Matta, Rebecca Lewis, Christopher Fellows, Gyula Diszhazi, Janos Almassy, Nicolai Miosge, James E. Dixon, Marcos C. Uribe, Sean May, Szilard Poliska, Richard Barrett-Jolley, Erin Henslee, Fatima H. Labeed, Michael P. Hughes, Ali Mobasheri |
| Rok vydání: |
2020 |
| DOI: |
10.21203/rs.3.rs-106355/v1 |
| Popis: |
Chondrogenic progenitor cells (CPCs) may be used as an alternative source of cells with potentially superior chondrogenic potential compared to mesenchymal stem cells (MSCs), and could be exploited for future regenerative therapies targeting articular cartilage in degenerative diseases such as osteoarthritis (OA). In this study, we hypothesised that CPCs derived from OA cartilage may be characterised by a distinct channelome. First, a global transcriptomic analysis using Affymetrix microarrays was performed. We studied the profiles of those ion channel and transporter families that may be relevant to chondroprogenitor cell physiology. Following validation of the microarray data, we examined the role of calcium-dependent potassium channels in CPCs and observed functional large conductance calcium-activated potassium channels (BK) involved in the maintenance of chondroprogenitor phenotype. In line with our very recent results, we found that the KCNMA1 gene was upregulated in CPCs and observed currents that could be attributed to the BK channel in both cell types. Through characterisation of their channelome we demonstrate that CPCs are a distinct cell population but are highly similar to MSCs in many respects. This work adds key mechanistic data to the in-depth characterisation of CPCs and their phenotype in the context of cartilage regeneration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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