Melanopsin mRNA in the Iris of Red-Eared Slider Turtles (Trachemys scripta elegans)
| Autor: | Lauren P. Hartnett, James R. Dearworth, Laura A. Goldberg, Melroy Dsouza, David R. Perlow, Cathryn L. Kubera, Brian P. Selvarajah, Michael J. Chejlava, Sze Cheng, Joseph Sherma, Scott J. Esckilsen, Christopher R. Anderson, Eric S. Ho |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Melanopsin Gene isoform Retina urogenital system fungi Retinal In situ hybridization Anatomy Biology urologic and male genital diseases Molecular biology Pupil 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure chemistry medicine Animal Science and Zoology Photopigment cardiovascular diseases sense organs Iris (anatomy) Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Herpetology. 51:538-551 |
| ISSN: | 0022-1511 |
| DOI: | 10.1670/16-046 |
| Popis: | — The iris in Red-Eared Slider Turtles (Trachemys scripta elegans) is intrinsically photosensitive, slowly constricting the pupil when exposed to light. We hypothesized that one of the photopigments involved in the photomechanical response (PMR) is melanopsin (Opn4). The purpose of this project was to determine whether mRNA of Opn4 is present in their iris. After eyes were dissected from animals, we extracted iris tissues to carry out fluorescent in situ hybridization. Comparison of fluorescence from images revealed significant labeling in the iris for two melanopsin isoforms (Opn4x and Opn4m). We then carried out quantitative real-time PCR to identify relative expression levels of mRNA. Expressions by both isoforms in the iris were significant. In contrast to the retina, which had higher levels of Opn4m than Opn4x, the iris had higher levels of Opn4x than Opn4m. We also carried out Carr–Price reactions on tissue extracts, which were stabilized by hydroxylamine, and detected retinal oxime in the ... |
| Databáze: | OpenAIRE |
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