Development of a double monoclonal antibody sandwich ELISA test forVerticillium dahliaeKleb
| Autor: | Seher Benlioglu, K. Benlioglu, Fatima Yucel, Aynur Başalp, Selma Öztürk |
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| Rok vydání: | 2005 |
| Předmět: |
Veterinary medicine
food.ingredient medicine.drug_class Immunology food and beverages Fungi imperfecti Biology Verticillium biology.organism_classification Monoclonal antibody Microbiology Serology food medicine Agar Verticillium dahliae Verticillium wilt Agronomy and Crop Science Pathogen Food Science |
| Zdroj: | Food and Agricultural Immunology. 16:283-291 |
| ISSN: | 1465-3443 0954-0105 |
| Popis: | Verticillium dahliae causes wilt diseases in a wide range of horticultural and field crops in many parts of Turkey. In the Aegean region it has been a serious problem in cotton and vegetables for many years. More recently, it has become a major problem for olive growers in relatively newly established orchards. The fact that the only possible control methods of Verticillium wilt disease is the use of healthy and pathogen free propagating material has directed us to produce monoclonal antibodies against V. dahliae in order to apply rapid, sensitive and reliable serological detection methods. Verticillium spp. isolates were obtained from olive plantations, as well as from cotton and tomato fields in the Aegean and Marmara Regions. All suspected isolates were obtained as V. dahliae after determining microscopic morphological characteristics and pathogenicity tests on cotton seedlings. Immunizing antigens were prepared by three different methods including surface washing system, czapek dox agar and g... |
| Databáze: | OpenAIRE |
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