An improved method for the mass screening of hemoglobin diseases

Autor:	F.D. Schap, Moira Breen, H.G. Weinstein
Rok vydání:	1968
Předmět:	Chromatography Resolution (mass spectrometry) Chemistry Ionic strength Negative charge Hemoglobin synthesis Hemoglobin variants Improved method Hemoglobin Biochemistry Mass screening
Zdroj:	Biochemical Medicine. 2:35-40
ISSN:	0006-2944
DOI:	10.1016/0006-2944(68)90005-7
Popis:	A simple modification of the micro-electrophoresis procedure for separation and identification of hemoglobin variants is described. Changing the buffer from barbital pH 8.6 to Tris-glycine pH 9.3 at the same ionic strength increases the negative charge of the hemoglobins, and provides an environment of positively-charged counter ions (glycinium) for them, thus increasing their relative mobility and resolution. The method is of value in detecting hemoglobinopathies which otherwise may be missed. The procedure is suitable for routine clinical hospital laboratories and is a valuable research tool for the rapid screening of samples in studying the genetics of hemoglobin synthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::64f2dc8aa5a99bcc394d47f20966dabf https://doi.org/10.1016/0006-2944(68)90005-7 Zobrazit plný text záznamu