Novel arsenic biosensor 'POLA' obtained by a genetically modified E. coli bioreporter cell
| Autor: | L.A. Pola-López, Rubén Grajales-Coutiño, Agustino Martínez-Antonio, María Celina Luján-Hidalgo, G. Anzueto-Sanchez, J.H. Castañón González, J.L. Camas-Anzueto, Víctor Manuel Ruíz-Valdiviezo |
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| Rok vydání: | 2018 |
| Předmět: |
Optical fiber
Analytical chemistry chemistry.chemical_element 02 engineering and technology 01 natural sciences Signal Green fluorescent protein law.invention chemistry.chemical_compound law Materials Chemistry Electrical and Electronic Engineering Instrumentation Arsenic Arsenite 010401 analytical chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Biophysics Bioreporter 0210 nano-technology Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 254:1061-1068 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.08.006 |
| Popis: | In this paper, we present a new vector named vector total POLA (VtP), which senses arsenite as input signal, and has a signal amplifier in order to express a green fluorescent protein as a response. This vector functions in the bacterium E. coli K-12. It is demonstrated that the fluorescence signal at 530 nm of the living arsenic biosensor increases linearly with elapsed time and as arsenite concentration increases. The range has been proven to detect from 5 to 140 μg/l of arsenite. An experiment was carried out to determine the kinetic response of the bacterial biosensor as a function of arsenite concentration for 12 h, which confirmed that the development of the new vector produce a well-defined linear response as fluorescence. This measurement can be carried out in as little as 1 h at different concentrations of arsenite. A preliminary test where the bacterial biosensor was coupled with an optical fiber was performed in order to investigate the possibility of its integration as an optical fiber biosensor in the near future. The optical power output also presented a linear dependency on arsenite concentration. |
| Databáze: | OpenAIRE |
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