| Autor: |
Edmond H. Fischer, Diana I. Lurie, Flavio F. Solca, Santosh Kumar, Curtis D. Diltz, Richard S. Johnson, Edwin W. Rubel |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 269:27559-27565 |
| ISSN: |
0021-9258 |
| Popis: |
The identification, purification, and biochemical characterization of specific markers for neuroglial cells in the central nervous system is an essential step toward a better understanding of the function of glial cells. This manuscript reports the identification and purification of a neuroglia-associated protein (NAP-185) with an apparent molecular mass of 185 kDa. While its expression is not restricted to the brain, it was first identified in a specific subpopulation of glial cells when chick brain stem sections were analyzed with an affinity-purified rabbit antiserum raised against the catalytic domain of the T-cell protein tyrosine phosphatase. This 185-kDa antigen was purified to apparent homogeneity and confirmed to be responsible for the neuroglial staining observed. In spite of its immunological relation to T-cell protein tyrosine phosphatase, purified NAP-185 failed to display tyrosine phosphatase activity. The primary sequence of five NAP-185-derived peptides shows that this protein has not yet been characterized and that it is possibly related to AP180, a clathrin-associated protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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