Effect of irradiation on the detection of bacterial DNA in contaminated food samples by DNA hybridization

Autor:	T.F. Rowe, K.J. Towner
Rok vydání:	1994
Předmět:	biology Microorganism DNA–DNA hybridization Contamination biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound chemistry Radicidation Nucleic acid Irradiation Food science DNA Bacteria
Zdroj:	Letters in Applied Microbiology. 18:171-173
ISSN:	1472-765X 0266-8254
DOI:	10.1111/j.1472-765x.1994.tb00837.x
Popis:	A membrane-based RNA hybridization technique was used in a model system to examine the effect of irradiation treatment on the detection of bacterial contamination in foodstuffs. Although hybridization signals were reduced compared with otherwise identical unirradiated food samples, artificial contamination levels in excess of 10 5 cfu per test could be distinguished in 12 of the 13 foods examined following the irradiation process. In no case were viable bacteria detected following irradiation treatment
Databáze:	OpenAIRE
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