Genomic Profile of rIR and rIRR in Type 2 Diabetes Mellitus Rat Model toward Effect of Goat Milk CSN1S2 Protein

Autor:	H. N. Meidinna, S. H. Tambunan, F. Fatchiyah, R. N. Rohmah
Rok vydání:	2019
Předmět:	History medicine.medical_specialty Endocrinology Internal medicine Rat model Genomic Profile medicine Type 2 Diabetes Mellitus Biology Computer Science Applications Education
Zdroj:	Journal of Physics: Conference Series. 1374:012046
ISSN:	1742-6596 1742-6588
DOI:	10.1088/1742-6596/1374/1/012046
Popis:	Diabetes mellitus is metabolic disorder with hypergycemia condition. Diabetes meilitus is commonly divided into two types, type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). The research focus was to determine the effect of CSN1S2 protein from Etawah Crossbred goat milk on DNA sequence of rIR exon 17 and rIRR exon 2 in T2DM rat model. The experimental rats were divided into four main groups, namely control group, diabetes group, control with CSN1S2 protein treatment group, and diabetes with CSN1S2 protein treatment group. We used 375, 750 and 1500 mg/kg BW of goat milk CSN1S2 protein for the treatment. The DNA was extracted from the rat liver, then amplified with a spesific primer of rIR and rIRR genes. The results of the amplification will be sequenced by using ABI 3730xl DNA Sequencer. The CSN1S2 protein treatment in rIR gene exon 17 did not affect a specific DNA sequence alteration. The diabetic rats treated with 375, 750 and 1500 mg/kg BW of CSN1S2 protein had the nucleotide changed to normal (3950A→3950G). Therefore, the CSN1S2 protein may have the function in triggering the DNA repair system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::64469627d4910ae1333b720ba1cb5149 https://doi.org/10.1088/1742-6596/1374/1/012046 Zobrazit plný text záznamu