Influence of Norepinephrine and Catecholamine-Depleting Agents on the Synthesis and Release of Prolactin and Growth Hormone1
| Autor: | Robert M. MacLeod, Ronald C. Pace, H. Fontham |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Estrous cycle
endocrine system medicine.medical_specialty Biology Reserpine Tyramine Prolactin Norepinephrine (medication) chemistry.chemical_compound Endocrinology Epinephrine chemistry Internal medicine medicine Catecholamine Incubation hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Endocrinology. 85:916-923 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-85-5-916 |
| Popis: | The in vitro incorporation of leucine- 4,5-3H into rat pituitary glands and its subsequent release into the incubation medium were studied. Quantitation of prolactin and growth hormone was by measurement of intensity of the bands obtained with the polyacrylamide gel technique without bioassay. Most of this newly synthesized prolactin is released into the medium and not retained within the gland. The addition of norepinephrine (10-6M) caused a 70–85% decrease in the amount of radioactive prolactin released by the gland during a 7-hr incubation. An accumulation of labeled prolactin occurred in the treated glands, indicating the primary action of norepinephrine was to decrease prolactin release. The accumulation, however, was not proportional to the degree of inhibition of release, indicating that prolactin synthesis was secondarily inhibited. An increase in the radioactive prolactin in the gland was detectable 30 min afterthe addition of norepinephrine and was statistically significant 30 min later. Similar... |
| Databáze: | OpenAIRE |
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