Messenger RNA profiling of rabbit quadriceps femoris after repeat injections of botulinum toxin: Evidence for a dynamic pattern without further structural alterations
| Autor: | Rafael Fortuna, Walter Herzog, David A. Hart |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology Physiology Adipokine 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Physiology (medical) Internal medicine medicine Messenger RNA business.industry Mesenchymal stem cell Muscle weakness Botulinum toxin Muscle atrophy Quadriceps femoris muscle 030104 developmental biology Endocrinology Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery Muscle contraction medicine.drug |
| Zdroj: | Muscle & Nerve. 57:487-493 |
| ISSN: | 0148-639X |
| DOI: | 10.1002/mus.25775 |
| Popis: | Introduction: Onabotulinum toxin-A (BoNT-A) is widely used clinically, but might cause adverse effects. Earlier studies showed repeat BoNT-A injections did not cause progressive atrophy or function loss. The purpose of this study was to assess the influence of repeat BoNT-A injections into rabbit muscle on subsequent molecular alterations. Methods: Twenty-two rabbits received 0, 1, 2, or 3 BoNT-A injections in the quadriceps femoris muscle at 3 month intervals and were sacrificed 6 months after the last injection. Aliquots of the injected and contralateral muscle were frozen, and the total RNA quantified. Results: Total RNA/mg wet weight tissue was significantly elevated compared to control following 1, 2 or 3 BoNT-A injections. Analysis of mRNA levels for inflammatory molecules, proteinases, adipokines, and mesenchymal stem cells were elevated with increasing BoNT-A injections in injected and contralateral leg. Discussion: Future studies should focus on the safety and possible complications of repeat BoNT-A treatments. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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