CLINICAL APPLICATION OF PLASMA PROTEIN C DETERMINATION

Autor: RM Bertina, George Zhu, AW Broekmans
Rok vydání: 2021
Předmět:
Zdroj: Universal Journal of Pharmaceutical Research.
ISSN: 2456-8058
DOI: 10.22270/ujpr.v5i6.509
Popis: Objective: Protein C, a vitamin K-dependent coagulation factor, is involved in blood coagulation. Activated protein C inactivates Va and VIIIa and stimulates fibrinolysis. In this process, protein S serve as an important factor for activated protein C. Furthermore, excess protein S drives cancer cell proliferation and cell survival through oncogenic receptor Axl(Anexelekto). We determined ranges of protein C both in healthy individuals and distinct hospitalized patients. Methods: A total of 100 patients with different diagnostic diseases and 50 healthy individuals were included in their plasma protein C determination. A rabbit antibody against human protein C was used for the quantitative estimation of plasma protein C antigen by using rocket immunoassay. Results: In healthy individuals protein C antigen (PC:Ag) ranges o.6439- 1.4752 µ/ml. The mean coefficient of variation (CV) of length of rocket was calculated to be 12.45%. PC:Ag within laboratory variation was 11.47%. Plasma protein C antigen was destroyed at 56℃ for 30 minutes, whereas no significant decrease of protein C was found at 4℃ refrigerator for one week. Conclusion: The results showed that plasma protein C antigen was considerably high in 22 diabetes mellitus. On the other hand, the PC:Ag was significantly decreased in 19 liver cirrhosis(p< 0.001) and was positively correlation with serum albumin levels(p< 0.05). In 20 acute leukemias, on the average,there was slightly lower values in PC:Ag, and accompanied with significant decrease of PC:Ag in 5 M5 subtype and in 9 hyper- leukocytes acute leukemias. However, the 3 acute promyelocytic leukemia (APL) with overt laboratory picture of DIC(disseminated intravascular coagulation) had protein C concentration no lower than the remaining 2 patients with infectious DIC, which suggested the coagulopathy in APL might be due to mechanisms different from other forms of DIC. Peer Review History: Received 17 November 2020; Revised 12 Decembe; Accepted 1 January, Available online 15 January 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Comments of reviewer(s): Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Idoko Alexander, Caritas University, Enugu, Nigeria, idokoalexander1@gmail.com Dr. Rawaa Souhil Al-Kayali, Aleppo University, Syria, rawah67@hotmail.com Similar Articles: THE ASSOCIATION BETWEEN LEVELS OF HEPCIDIN, IRON STATUS AND MICRO-INFLAMMATION MARKERS AMONG HAEMODIALYSIS COUMARIN ANALOGUES AS A POTENTIAL INHIBITOR OF LEISHMANIASIS: A MULTI-TARGETING PROTEIN INHIBITION APPROACH BY MOLECULAR DOCKING VITAMIN A, RETINOIC ACID AND TAMIBAROTENE, A FRONT TOWARD ITS ADVANCES: A REVIEW
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