Preparation and Culturing of Human Primary Vascular Cells
| Autor: | Ingvar Hakon Olafsson, Finnbogi R. Thormodsson, Dadi Th. Vilhjalmsson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pathology
medicine.medical_specialty Vascular smooth muscle Amyloid biology business.industry Leptomeninges medicine.disease Umbilical cord 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Cystatin C Cerebral cortex Cell culture 030220 oncology & carcinogenesis medicine biology.protein Cerebral amyloid angiopathy business 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493978151 |
| DOI: | 10.1007/978-1-4939-7816-8_21 |
| Popis: | Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer's disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs: First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVEC), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described. |
| Databáze: | OpenAIRE |
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