| Autor: |
Naseem Mushtaq, Norrice M. Liu, Lisa Miyashita, Michele Padovan, Jonathan Grigg |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Respiratory Infections. |
| DOI: |
10.1183/1393003.congress-2017.pa3321 |
| Popis: |
Introduction: Increasing evidence suggests a link between particulate matter (PM) and cystic fibrosis disease severity 1 . Pseudomonas aeruginosa (PA) chronically colonises the lungs of CF patients. Pyocyanin (PYC), a major PA virulence factor is often recovered from CF lungs in large quantities. We previously reported that PM exposure alters the PA secretion profile, inducing cytotoxicity to lung cells 2 . Aim: We sought to investigate whether PYC production is increased following PM exposure. Methods: PA strain 14 was grown overnight in LB broth. Control PBS or diesel particulate matter (DPM) was added to dilutions of PA14 cultures (10µg/ml) and incubated for 6 hrs. Production was measured using a quantitative assay in which PYC was extracted from culture supernatants (SN) with chloroform and HCl. Absorbance was measured (520nm). Results are expressed as average PYC production (µg/ml SN) from 3 experiments and analysed by unpaired T-test. Results: A significant increase in pyocyanin production was oserved in DPM exposed cultures (p Conclusion: PA exposure to DPM results in increased PYC production. As PYC plays an important role in PA infection, this may contribute to the association between exposure to particulate matter and disease severity in CF patients. 1 Psoter K et al Ann Am Thor Soc 12(3). 2 Mushtaq N et al ERJ 2013. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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