Synthesis of oligonucleotide-functionalized magnetic nanoparticles and study on theirin vitrocell uptake

Autor:	Kerstin Wagner, Joachim H. Clement, Michael Röder, Armin Rex Kautz, Katharina Pachmann, Manuela Schwalbe, Matthias Schnabelrauch
Rok vydání:	2004
Předmět:	Chemistry Endosome Oligonucleotide Nanoparticle General Chemistry In vitro Inorganic Chemistry chemistry.chemical_compound Dextran Biochemistry Transmission electron microscopy Biophysics Magnetic nanoparticles Iron oxide nanoparticles
Zdroj:	Applied Organometallic Chemistry. 18:514-519
ISSN:	0268-2605
DOI:	10.1002/aoc.752
Popis:	Carboxymethyl dextran (CMD) with varying degrees of substitution was prepared and used as biocompatible coating for magnetic iron oxide nanoparticles. An oligonucleotide (19-mer) was coupled to the CMD-coated particles as a model compound for DNA fragments. Transmission electron microscopy investigations on the cellular uptake of the particles by different tumor cell lines demonstrated that both the CMD-coated and the oligonucleotide-coupled particles are internalized by the cells and deposited in cellular endosomes. The nanoparticles prepared have potential applications in tumor diagnosis and therapy.
Databáze:	OpenAIRE
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