Novel plasmonic platform for ultra-sensitive detection and diagnostics
| Autor: | Julian Borejdo, Bo W. Laursen, Ilkay Bora, Sangram Raut, Ryan Rich, Tanya Shtoyko, Thomas Just Sørensen, Sebastian Requena, Zygmunt Gryczynski, Ignacy Gryczynski |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Analytical chemistry Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Human serum albumin 01 natural sciences Fluorescence 0104 chemical sciences Metal Extinction (optical mineralogy) visual_art visual_art.visual_art_medium medicine Emission spectrum 0210 nano-technology Luminescence Excitation Plasmon medicine.drug |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2221923 |
| Popis: | In this report, we describe a plasmonic platform with silver fractals for metal enhanced fluorescence (MEF) measurements. When a dye containing surface was brought into contact with silver fractals, a significantly enhanced fluorescence signal from the dye was observed. Fluorescence enhancement was studied with the N -methyl-azadioxatriangulenium chloride salt (Me-ADOTA.Cl) in PVA films made from 0.2 % PVA (w/v) solution spin-coated on a clean glass coverslip. The Plasmonic Platforms (PP) was assembled by pressing together silver fractals on one glass slide and a separate glass coverslip spin-coated with a uniform Me-ADOTA.Cl in PVA film. In addition, we also tested the ADOTA labeled human serum albumin (HSA) deposited on a glass slide for potential PP bioassay applications. Using the new PP, we could achieve more than 20-fold fluorescence enhancement (bright spots) accompanied by decrease in fluorescence lifetime. The experimental results were used to calculate the extinction (excitation) enhancement factor (GA) and fluorescence radiative rate enhancements factor (GF). No change in emission spectrum was observed for a dye with and without contact with fractals. Our studies indicate that this type of PP can be a convenient approach for constructing assays utilizing metal enhanced fluorescence (MEF) without the need for depositing the material directly on metal structures platforms. |
| Databáze: | OpenAIRE |
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