| Popis: |
In humidity-controlled atmospheres above water or different concentrations of aqueous NaCl solutions at 20-22 C, mature apothecia on dry, infected alfalfa leaves (Medicago sativa) rehydrated and discharged ascospores of Leptotrochila medicaginis more abundantly near 99.5 and 100% RH and less abundantly at progressively lower RH values; RH limits for discharge were 98.0% at 14.4 and 15.5 C and 97.5% at 20-22 and 26-28 C. On glass in a moist chamber (∼100% RH) and on fresh water agar (WA) at 20-25 C, discharged ascospores swelled and formed short germ tubes within 4 hr, which lengthened to spore width (approximately 5 μm) within 6 hr (.) |
