Sulfurimonas crateris sp. nov., a facultative anaerobic sulfur-oxidizing chemolithoautotrophic bacterium isolated from a terrestrial mud volcano
| Autor: | G. B. Slobodkina, D. S. Kopitsyn, Elizaveta A. Bonch-Osmolovskaya, N M Ratnikova, V. V. Kevbrin, Alexander I. Slobodkin, Alexander Y. Merkel |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Thiosulfate Epsilonproteobacteria biology Strain (chemistry) chemistry.chemical_element General Medicine biology.organism_classification 16S ribosomal RNA 010603 evolutionary biology 01 natural sciences Microbiology Sulfur 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Sulfurimonas Botany Ecology Evolution Behavior and Systematics Bacteria Mesophile |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 70:487-492 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A novel mesophilic facultative anaerobic bacterium, strain SN118T, was isolated from a terrestrial mud volcano in Taman Peninsula, Russia. The cells were Gram-negative, motile, short, straight or curved rods with a single polar flagellum. Growth was observed at 5-40 °C (optimum, 30 °C) and pH 5.5-9.5 (optimum, pH 8.0). Growth of strain SN118T was observed in NaCl concentrations ranging from 0.5 to 8.0 % (w/v) with an optimum at 2.0-3.0 % (w/v). The isolate grew chemolithoautotrophically with sulfide, elemental sulfur or thiosulfate as electron donor, oxygen, nitrate or nitrite as an electron acceptor and CO2/HCO3 - as a carbon source. Molecular hydrogen or organic substances did not support growth. Nitrate was reduced to N2. The dominant fatty acids were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The total size of the genome of the novel isolate was 2 209 279 bp and the genomic DNA G+C content was 38.8 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate belonged to the genus Sulfurimonas and was most closely related to Sulfurimonas denitrificans DSM 1251T (96.74 %). Based on its physiological properties and results from phylogenetic analyses, including average nucleotide identity and in silico DNA-DNA hybridization values, the isolate is considered to represent a novel species of the genus Sulfurimonas, for which the name Sulfurimonas crateris sp. nov. is proposed. The type strain is SN118T (=DSM 109248T=VKM B-3378T). |
| Databáze: | OpenAIRE |
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