| Autor: |
Ravi Tiwari, Mustapha Trabelsi, Fatma Tajini, Moncef Mrabet, Ridha Mhamdi, Mohamed Elarbi Aouani |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
European Journal of Agronomy. 22:209-216 |
| ISSN: |
1161-0301 |
| DOI: |
10.1016/j.eja.2004.02.006 |
| Popis: |
The rhizobia strain R. gallicum bv. gallicum 8a3 was isolated from root nodules of Phaseolus vulgaris cultivated in Tunisian soils. This strain was selected on the basis of its high symbiotic effectiveness in laboratory conditions. In order to assess its ability to compete indigenous rhizobia, this strain was labelled with gusA gene. Conservation of initial effectiveness and competitiveness by transconjugants was tested. A transconjugant was introduced in three soil-core microcosms originating from different geographical and agronomic regions. Nodulation monitoring showed that the labelled transconjugant was able to occupy more than 90% of nodules at 30 days after inoculation. The nodule occupancy by the introduced strain was high even in the soil sample of Mateur which showed an MPN value of 103 rhizobia/g of dry soil. A significant improvement of plant productivity by inoculation was observed with the three soil samples in green house. Field inoculation with the parental strain showed a significant increase in nodule number, pod number and seed dry weight. The improvement of plant productivity in green house or in field conditions was equal or better than nitrogen fertilisation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect