| Popis: |
Enzymes have several applications and potential, in addition to being an alternative for industry because they have advantages over synthetic chemicals that release polluting and toxic by-products into the environment. The optimization of the production of enzymes that degrade cellulose is of great interest, and in order to increase the viability of its use, several studies have been selecting sources and identifying means of obtaining and evaluating the enzymatic activity of these enzymes. Microorganisms can be used in the recycling of waste or in the production of several products with high added value, including biomolecules of industrial importance. The low cost of this raw material allows the development of economically competitive and ecological processes. Coconut fiber is an abundant lignocellulosic material and a rich source of cellulose, hemicellulose, and lignin, presenting itself as a promising and low-cost raw material for the production of enzymes. This work aims to produce endoglucanase from microorganisms isolated from coconut fiber to produce second-generation ethanol. For this, the kinetic profile of the enzymatic complex containing cellulases was evaluated, analyzing the effects of moisture content, temperature, and pH during the solid-state fermentation of coconut fiber, using a microorganism isolated from the bagasse itself. The Statistica software was used to evaluate the enzyme's complex production parameters through Pareto charts and response surface plotting. The statistical results indicated that the best conditions of humidity, temperature, and pH were 80%, 30°C, and 3 with activities of 182 U/g endoglucanase in a time of 168 h. |
