High Prevalence of the I278T Mutation of the Human Cystathionine β-Synthase Detected by a Novel Screening Application

Autor:	H. G. Koch, Erik Harms, Ulrike Nowak-Goettl, Michael Linnebank, Ralf Junker, Anja Homberger
Rok vydání:	2001
Předmět:	biology business.industry Point mutation Metabolic disorder Homocystinuria Hematology medicine.disease Cystathionine beta synthase Loss of heterozygosity Genotype Immunology medicine biology.protein Allele business Allele frequency
Zdroj:	Thrombosis and Haemostasis. 85:986-988
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0037-1615951
Popis:	SummaryClassical homocystinuria due to cystathionine beta-synthase deficiency is one of the disorders revealing a high risk of thromboembolic events and vascular disease. This autosomal-recessively inherited metabolic disorder is considered to be rare with an estimated prevalence of 1:130,000 in the German population. In this study, we developed a novel multiplex PCR generating allele specific fragment lengths to analyse individual genotypes of the two most frequent cystathionine beta-synthase alterations, the I278T mutation, which is worldwide found on up to the half of homocystinuric alleles, and the adjacent polymorphism 844ins68. Screening of 200 unrelated German control subjects revealed a frequency of heterozygosity of 1.5% for I278T corresponding to a calculated frequency of homozygosity of 1:17.800. Our data indicate that homocystinuria due to cystathionine β-synthase deficiency is a frequently unrecognized disorder resulting in a high risk of thromboembolic events.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5f69d09f587154afa326c3f74ece522c https://doi.org/10.1055/s-0037-1615951 Zobrazit plný text záznamu