| Autor: |
Colin R. Howard, P.H. Russell, Kin-Chow Chang, Theophanis Kanellos, I D Sylvester |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Fish & Shellfish Immunology. 8:121-128 |
| ISSN: |
1050-4648 |
| DOI: |
10.1006/fsim.1997.0123 |
| Popis: |
Reporter genes are increasingly being used in order to assess new approaches to immunisation with nucleic acids. In the present work goldfish (Carassius auratusL.) received theEscherichia coliβ-galactosidase (β-gal) reporter gene under control of the immediate early promoter/enhancer of the human cytomegalovirus by intramuscular injection just anterior to the dorsal fin. β-gal activity was detected at the site of injection after 4 days. Antibody to β-gal was present in the sera of fish killed at 4 and 8 weeks after DNA injection. In parallel experiments with mice only half the mice were positive for antibody, this being mainly of the IgG2a isotype, at 4 weeks after injection of DNA into their anterior tibial muscles. This suggests goldfish can sero-convert more readily than mice within 4 weeks of DNA injection using the β-gal reporter gene. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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