Comparative Analysis of Pathogenicity Assays and PCR for Listeria monocytogenes Isolated from Animal, Raw Food and Environmental Sources in Korea

Autor:	Gunyong Park, Byungyeol Jung, Mi-Ok Song, Hyowon Jung, Sungmin Choi, Jihun Jung, Jib-Ho Lee, Young-Zoo Chae, Myunghun Lee, Sang-Hun Park, Seung-Hee Ryu, Sangmi Lee, Sun-Hee Park, Jung-Hun Kim, Hyun-Chul Kim, Younghee Oh
Rok vydání:	2013
Předmět:	Strain (chemistry) Listeria monocytogenes Vero cell Pulsed-field gel electrophoresis medicine Virulence Internalin General Medicine Primer (molecular biology) Biology medicine.disease_cause Gene Microbiology
Zdroj:	Advances in Microbiology. :191-199
ISSN:	2165-3410 2165-3402
DOI:	10.4236/aim.2013.32029
Popis:	Results of PCR with oligonucleotide primers were designed from the assembled panel of four potential virulence genes (two of internalin gene and two of transcriptional regulator gene). Most of the isolates including reference strains were reactive by PCR, whereas the other strains (No.80, 81, and 83) isolated from pork, were non-reactive by PCR. In particular, all pork isolates were PCR-negative for two primers (lmo2672 and 2821) sets tested. However, No.82 was positive for lmo1134 primer, and No.84 was positive for lmo2470 of pork isolates. It was observed that all Listeria monocytogenes (L. monocytogenes) penetrate Vero cells, although the invasion efficiency of each strain varied (between 0.5 and 18.9%). When compared in cell assay with PFGE, the results were shown that the mean invasion efficiency for lineage II isolate (2.6%) was significantly lower (ANOVA-test, p
Databáze:	OpenAIRE
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