Small Interfering RNA Nunchucks with a Hydrophobic Linker for Efficient Intracellular Delivery

Autor:	Cheol O. Joe, Jee Seon Kim, Sung Duk Jo, Hyejung Mok, Yoon Sung Nam
Rok vydání:	2013
Předmět:	Polyethylenimine Small interfering RNA Polymers and Plastics Bioengineering Polyelectrolyte Biomaterials chemistry.chemical_compound chemistry Materials Chemistry Biophysics Gene silencing Organic chemistry Linker DNA Intracellular Biotechnology Conjugate
Zdroj:	Macromolecular Bioscience. 14:195-201
ISSN:	1616-5187
DOI:	10.1002/mabi.201300291
Popis:	The high stiffness and low spatial charge density of siRNA limit the effectiveness of the electrostatic condensation of siRNA with cationic polyelectrolytes. Here, a facile method to stabilize nanoscale siRNA/polyelectrolyte complexes by introducing a reductively cleavable alkyl chain to siRNA as a hybrophobic linker of dimeric siRNA conjugates is reported. The increased length of the hydrophobic linker increases the intracellular translocation and gene silencing activity of the dimeric siRNA conjugates when they are complexed with linear polyethylenimine (LPEI). The results suggest that the introduction of a hydrophobic linker in the dimeric siRNA conjugates can facilitate the intracellular delivery of siRNA through effective condensation with cationic polyelectrolytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5f34f0dda4ced79e986212a53b415240 https://doi.org/10.1002/mabi.201300291 Zobrazit plný text záznamu Plný text