The nitroxide Tempol affords protection against ultraviolet radiation in a transgenic murine fibroblast culture model of cutaneous photoaging
| Autor: | Eric F. Bernstein, Francis P. Gasparro, Sung K. Kong, Douglas B. Brown, Tsunemichi Takeuchi, Jouni Uitto, B. C. Kwak |
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| Rok vydání: | 2001 |
| Předmět: |
Genetically modified mouse
chemistry.chemical_classification Reactive oxygen species biology Chemistry Photoaging Biological activity Dermatology Pharmacology medicine.disease Biochemistry Superoxide dismutase medicine.anatomical_structure Cell culture cardiovascular system medicine biology.protein Fibroblast Molecular Biology Elastin |
| Zdroj: | Experimental Dermatology. 10:55-61 |
| ISSN: | 0906-6705 |
| DOI: | 10.1034/j.1600-0625.2001.100107.x |
| Popis: | The generation of reactive oxygen species is among the various mechanisms by which ultraviolet radiation damages skin. Tempol, a superoxide dismutase analogue which readily penetrates cell membranes when administered exogenously, has been shown to provide protection against some forms of oxygen-dependent damage. In this study, we measured the ability of Tempol to protect against ultraviolet A- and ultraviolet B-induced damage, using a previously described transgenic mouse model of cutaneous photoaging. The ability of Tempol to prevent ultraviolet radiation-induced elastin promoter activation was determined in vitro. Tempol provided over 50% protection against ultraviolet B and over 70% protection against ultraviolet A as measured in our in vitro system. These results demonstrate the ability of the superoxide dismutase mimic, Tempol, to protect against ultraviolet induced elastin promoter activation. This compound could be a useful pharmacological agent to prevent cutaneous photoaging. |
| Databáze: | OpenAIRE |
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