Complete genome sequence of a recombinant isolate of yambean mosaic virus from Canavalia ensiformis

Autor:	Renan de Souza Cascardo, Lorenna L. Pires, José Evando A. Beserra, Poliane Alfenas-Zerbini, Lorhan L. Leal, F. Murilo Zerbini
Rok vydání:	2021
Předmět:	Genetics Whole genome sequencing Sanger sequencing biology Contig Potyviridae viruses Potyvirus General Medicine biology.organism_classification Genome DNA sequencing Open reading frame symbols.namesake Virology symbols Molecular Biology
Zdroj:	Virus Genes. 57:561-564
ISSN:	1572-994X 0920-8569
DOI:	10.1007/s11262-021-01864-7
Popis:	The complete genome sequence of a Brazilian isolate of yambean mosaic virus (YBMV) is presented. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing revealed the complete genome sequence of the YBMV-BRA-6 isolate, found in Canavalia ensiformis. The de novo contigs were assembled into a 9612 nucleotides (nt) long scaffold, excluding the 3'-terminal poly(A) tail, covering the complete genome. The genomic RNA contains an open reading frame (ORF) typical of members of the genus Potyvirus, family Potyviridae, encoding a large putative polyprotein of 3078 amino acids (aa) and a small overlapping PIPO ORF. Pairwise comparisons showed that the YBMV-BRA-6 isolate sequence shares 88.1% nt identity for the complete genome and 90.6% aa identity for the polyprotein with the YBMV-SR isolate. Phylogenetic analysis grouped both isolates together and close to bean common mosaic virus (BCMV). The polyprotein cleavage sites were predicted and a recombination event is described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5e9b05e2be6e7787b6ddff63e52db1a1 https://doi.org/10.1007/s11262-021-01864-7 Zobrazit plný text záznamu Full text from SpringerLink