Comparison of the results of intradermal test reactivity and serum allergen‐specific I g E measurement for M alassezia pachydermatis in atopic dogs

Autor: Glenn R. Frank, Willam E. Oldenhoff, Douglas J. DeBoer
Rok vydání: 2014
Předmět:
Zdroj: Veterinary Dermatology. 25:507
ISSN: 1365-3164
0959-4493
DOI: 10.1111/vde.12159
Popis: Background Malassezia pachydermatis is part of the normal flora of canine skin. Malassezia hypersensitivity is recognized as a trigger for clinical signs of atopic dermatitis (AD) in some dogs. Determinations of Malassezia hypersensitivity are often made with intradermal testing (IDT), which may have limited availability in a first-opinion veterinary practice. Hypothesis/objectives The purpose of this study was to compare immediate IDT reactivity to M. pachydermatis with results of an enzyme-linked immunosorbent assay (ELISA) designed to detect anti-Malassezia IgE. Animals Eighty-four dogs with a clinical diagnosis of AD. Methods Multi-allergen IDT was performed on all dogs. Serum testing for allergen-specific IgE against a panel of common environmental allergens and M. pachydermatis was performed by ELISA using the FceRIα receptor fragment as a detection reagent, with results reported as adjusted optical density (OD). A receiver operating characteristic (ROC) curve was used to analyse the results of the two tests. Results The median adjusted OD of the anti-Malassezia IgE ELISA for dogs reactive and nonreactive to M. pachydermatis on IDT was 0.137 and 0.024, respectively. Analysis of the ROC curve suggested a cut-off point for the anti-Malassezia ELISA that yielded a sensitivity of 77.0% and a specificity of 89% relative to IDT results. Conclusions and clinical importance Substantial agreement was demonstrated between IDT reactivity and anti-Malassezia IgE as detected by the FceRIα receptor reagent. Although correlation with a clinical diagnosis of Malassezia dermatitis was not attempted in this study, the results indicate that the ELISA may be used to demonstrate the presence of immediate-type Malassezia hypersensitivity in dogs with AD.
Databáze: OpenAIRE
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