Lipase/chitosan nanoparticle-stabilized pickering emulsion for enzyme catalysis
| Autor: | Huan Liu, Cuige Zhang, Chaofei Huo, Qingzhong Liu, Rongli Zhang, Sheng Xu, Yuan Liu, Zongcheng Miao, Chunmiao Xia, Minmin Weng |
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| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
biology technology industry and agriculture Nanoparticle Pickering emulsion Enzyme catalysis Catalysis Chitosan Hydrolysis chemistry.chemical_compound Colloid and Surface Chemistry chemistry Emulsion Materials Chemistry biology.protein Physical and Theoretical Chemistry Lipase Nuclear chemistry |
| Zdroj: | Colloid and Polymer Science. 300:41-50 |
| ISSN: | 1435-1536 0303-402X |
| Popis: | An oil-in-water (O/W) Pickering emulsion catalysis system was prepared using a self-assembled of lipase/chitosan nanoparticles (CRL/CS NPs). The CRL/CS NPs functioned as both a stabilizer and a catalyst, and every droplet of the emulsion was used as a microreactor for the reactants in the oil phase. Due to an adequate oil–water interface for the “lid” opening of lipase and rapid mass transfer in the CRL/CS NPs, the Pickering emulsion exhibited excellent enzymatic activity toward the hydrolysis of olive oil, achieving 72.80% and 99.62% hydrolysis rates after 15 min and 120 min, respectively. Immobilizing lipase in the self-assembled CS NPs endowed the lipase with exceptional recyclability and provided a shield to protect the enzyme from deactivation. |
| Databáze: | OpenAIRE |
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