Avidin expression does not increase speed of kill of Spodoptera litura by baculovirus
| Autor: | L. N. Gatehouse, Joanne Poulton, Ngaire P. Markwick, John T. Christeller |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
animal structures
biology viruses fungi Insect cell culture Nuclear Polyhedrosis Virus Spodoptera litura Horticulture Spodoptera biology.organism_classification Molecular biology Virus Green fluorescent protein Autographa californica Insect Science parasitic diseases biology.protein Agronomy and Crop Science Avidin |
| Zdroj: | New Zealand Plant Protection. 56:194-200 |
| ISSN: | 1179-352X 1175-9003 |
| DOI: | 10.30843/nzpp.2003.56.6042 |
| Popis: | A disarmed (polh-minus) Autographa californica nuclear polyhedrosis virus-based baculovirus expressing green fluorescent protein (GFP) on the strong late promotor p10 was constructed. A second virus was made which also expressed the biotin-binding protein, avidin, from the strong late promotor polh. Both viruses caused strong fluorescence in Spodoptera frugiperda cells in insect cell culture. Seven-day-old larvae of Spodoptera litura were inoculated by microinjection with GFP virus, GFP+avidin virus or tissue culture medium (control). Mortality, growth rate and the development of green fluorescence were followed and after death the larvae were analysed for GFP and avidin content. All virus-injected larvae developed strong fluorescence. However, no significant differences in growth or mortality were seen between the larvae injected with virus expressing and not expressing avidin. Both GFP and avidin accumulated to high levels in the larvae. The expression of avidin did not change the level of expression of GFP. |
| Databáze: | OpenAIRE |
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