Screening of biologically important Zn 2+ by a chemosensor with fluorescent turn on - off mechanism
| Autor: | Venkata Nikhil Raj M, Surbhi Jain, Monika Sheoran, Diksha Gupta, Tanveer Khan, Sunil G. Naik |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
010405 organic chemistry Chemistry 010402 general chemistry Photochemistry 01 natural sciences Binding constant Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Analytical Chemistry Ion Proton NMR Titration Selectivity Instrumentation Zinc ion binding Spectroscopy |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 189:176-182 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2017.08.017 |
| Popis: | Reported herein the synthesis, characterization and biologically important zinc ion binding propensity of a weakly fluorescent chemosensor, 4-methyl-2,6-bis((E)-(2-(4-phenylthiazol-2-yl)hydrazono)methyl)phenol (1). 1H NMR spectroscopic titration experiment reveals the binding knack of 1 to the essential Zn2+. The photo-physical studies of 1 exhibit an enhancement in the fluorescence by several folds upon binding with the zinc ions attributed to PET-off process, with a binding constant value of 5.22×103M-1. 1 exhibits an excellent detection range for Zn2+ with lower detection limit value of 2.31×10-8M. The selectivity of 1 was studied with various mono and divalent metal cations and it was observed that most cations either quenches the fluorescence or remains unchanged except for Cd2+, which shows a slight enhancement in fluorescence intensity of 1. The ratiometric displacement of Cd2+ ions by Zn2+ ions shows an excellent selectivity towards in-situ detection of Zn2+ ions. Photo-physical studies also support the reversible binding of 1 to Zn2+ ions having on and off mechanism in presence of EDTA. Such recognition of the biologically important zinc ions finds potential application in live cell imaging. |
| Databáze: | OpenAIRE |
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