Ursolic Acid Treatment Alleviates Diabetic Kidney Injury By Regulating The ARAP1/AT1R Signaling Pathway
| Autor: | Ling-Xu Lu, Qiuling Fan, Lulu Li, Xu Cao, Li Xu, Tian-Kui Ma, Xu Wang, Xin Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
medicine.medical_specialty Creatinine Angiotensin II receptor type 1 business.industry NOX4 Glomerulosclerosis 030209 endocrinology & metabolism 030204 cardiovascular system & hematology medicine.disease medicine.disease_cause Angiotensin II 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology chemistry Fibrosis Internal medicine Internal Medicine Renal fibrosis Medicine business Oxidative stress |
| Zdroj: | Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 12:2597-2608 |
| ISSN: | 1178-7007 |
| DOI: | 10.2147/dmso.s222323 |
| Popis: | Purpose This study aimed to investigate whether ursolic acid (UA) mitigates renal inflammation, oxidative stress and fibrosis by regulating the angiotensin II type 1 receptor-associated protein (ARAP1)/angiotensin II type 1 receptor (AT1R) signaling pathway and subsequently alleviating renal damage. Methods db/db mice were divided randomly into a diabetic nephropathy (DN) group and a UA treatment group. Light microscopy and electron microscopy were used to observe pathological changes in renal tissues. Immunohistochemistry (IHC) was employed to examine changes in the expression of ARAP1, AT1R, 8-hydroxydeoxyguanosine (8-OHdG), NADPH oxidase 2 (NOX2), the extracellular matrix protein fibronectin (FN), IL-1β and IL-18 in renal tissues. Western blotting and RT-qPCR were used to detect the respective changes in the protein and mRNA levels of ARAP1, AT1R, NOX4, NOX2, transforming growth factor-β1 (TGF-β1), FN, collagen IV, IL-1β and IL-18 in renal tissues and mesangial cells. In addition, immunofluorescence staining was employed to examine changes in FN and NOX2 expression in mesangial cells. Results UA treatment effectively reduced the body weights and blood glucose levels of db/db mice (p |
| Databáze: | OpenAIRE |
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