MOLECULAR DETECTION OF INFECTION IN TOTAL KNEE ARTHROPLASTY: A CLINICAL CORRELATION

Autor:	Robert E. Booth, Tanya A. Gardner, Rocky S. Tuan, Marc J. Levine, Brian D. Mariani
Rok vydání:	1999
Předmět:	medicine.medical_specialty Microbiological culture business.industry medicine.medical_treatment Total knee arthroplasty Periprosthetic Clinical correlation Arthroplasty law.invention Surgery law medicine Synovial fluid Orthopedics and Sports Medicine Implant business Polymerase chain reaction
Zdroj:	Journal of Musculoskeletal Research. :93-107
ISSN:	1793-6497 0218-9577
DOI:	10.1142/s0218957799000117
Popis:	Total knee arthroplasty patients displaying symptoms consistent with either implant biomechanical failure or periprosthetic infection, and requiring revision surgery, had synovial fluid aspirated from the affected joint and subjected to standard microbiological culture testing for bacterial infection. In this study, an aliquot of each synovial fluid specimen was analyzed for infection using the rapid, sensitive molecular assay of polymerase chain reaction (PCR), designed to selectively amplify bacterial deoxyribonucleic acid. Decisions concerning surgical procedures performed during revision surgery were based entirely on clinical criteria, including standard preoperative evaluation for infection, independent of PCR results. In total, on the basis of pre- and/or intraoperative clinical data in conjunction with postoperative information, septic failure was determined in 31 of 50 patients, all of whom tested positive using the PCR assay. This correlative study validates the application of the PCR assay for clinical evaluation of arthroplasty infections.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5bd9f7e5095b222df5e6a9aec8417967 https://doi.org/10.1142/s0218957799000117 Zobrazit plný text záznamu