Water soluble steroid conjugates II. Metabolism of prednisone-21-glycinate and of cortisol-21-sulfate in the perfused dog liver
| Autor: | U. Volkwein, P. Berle, K. D. Voigt, J. Tamm |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
Pharmacology
medicine.medical_specialty medicine.medical_treatment Metabolite Organic Chemistry Clinical Biochemistry Metabolism Biochemistry Adrenosterone Steroid chemistry.chemical_compound Endocrinology chemistry Prednisone Internal medicine medicine Prednisolone Cortisone Molecular Biology hormones hormone substitutes and hormone antagonists medicine.drug Conjugate |
| Zdroj: | Steroids. 2:279-295 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(63)90006-0 |
| Popis: | Isolated livers of male dogs were perfused with human blood containing prednisone-21-glycinate, prednisolone, cortisol-21-sulfate or cortisol, respectively. The metabolic integrity of the organ was checked by means of enzyme estimations in the perfused medium. The following results have been obtained: 1. Prednisone-21-glycinate: Prednisolone and prednisone have been found in the fraction of free steroids. In the conjugate fraction two steroid sulfates were detected, probably conjugated at C21. 2, Prednisolone: In the fraction of free steroids prednisolone, prednisone, cortisol, and cortisone were characterized. The conjugate fraction contained two steroid sulfates with the characteristics of delta-1,4-dienes. Two other conjugates showed typical reactions of delta-4-5-ketosteroids 3. Cortisol-21-sulfate: The metabolism of this steroid conjugate was very slow compared with free cortisol. Only small amounts of cortisol were detectable in the fraction of free steroids. In the conjugate fraction cortisol-21-sulfate and Reichstein's compound E were characterized. 4. Cortisol: Cortisol, cortisone, adrenosterone, and6β, 11β-dihydroxy-androst-4-ene-3,17-dione were characterized as free steroids. Tetrahydrocortisone-glucuronide was the only conjugated metabolite. In all experiments a varying number of metabolites could not be characterized further. |
| Databáze: | OpenAIRE |
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