Intermediate-term memory in Aplysia involves neurotrophin signaling, transcription, and DNA methylation
| Autor: | Qizong Yang, David Castillejos, Robert D. Hawkins, Anagha Nagaraj, Caleb J. Bostwick, Andrea B. Kohn, Igor Antonov, Leonid L. Moroz |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology RNA methylation Cognitive Neuroscience biology.organism_classification Sensory neuron 03 medical and health sciences Cellular and Molecular Neuroscience 030104 developmental biology 0302 clinical medicine Neuropsychology and Physiological Psychology medicine.anatomical_structure Transcription (biology) Aplysia DNA methylation Synaptic plasticity medicine Epigenetics Intermediate-term memory Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Learning & Memory. 25:620-628 |
| ISSN: | 1549-5485 |
| Popis: | Long-term but not short-term memory and synaptic plasticity in many brain areas require neurotrophin signaling, transcription, and epigenetic mechanisms including DNA methylation. However, it has been difficult to relate these cellular mechanisms directly to behavior because of the immense complexity of the mammalian brain. To address that problem, we and others have examined numerically simpler systems such as the hermaphroditic marine mollusk Aplysia californica. As a further simplification, we have used a semi-intact preparation of the Aplysia siphon withdrawal reflex in which it is possible to relate cellular plasticity directly to behavioral learning. We find that inhibitors of neurotrophin signaling, transcription, and DNA methylation block sensitization and classical conditioning beginning ∼1 h after the start of training, which is in the time range of an intermediate-term stage of plasticity that combines elements of short- and long-term plasticity and may form a bridge between them. Injection of decitabine (an inhibitor of DNA methylation that may have other actions in these experiments) into an LE sensory neuron blocks the neural correlates of conditioning in the same time range. In addition, we found that both DNA and RNA methylation in the abdominal ganglion are correlated with learning in the same preparations. These results begin to suggest the functions and integration of these different molecular mechanisms during behavioral learning. |
| Databáze: | OpenAIRE |
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