A new approach on astaxanthin extraction via acid hydrolysis of wet Haematococcus pluvialis biomass
| Autor: | Rafael Bruno Vieira, André Bellin Mariano, Henrique Vechio |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Haematococcus pluvialis Chromatography biology Central composite design Chemistry 010604 marine biology & hydrobiology Extraction (chemistry) Plant Science Aquatic Science biology.organism_classification 01 natural sciences Solvent Hydrolysis chemistry.chemical_compound Astaxanthin Acetone Acid hydrolysis 010606 plant biology & botany |
| Zdroj: | Journal of Applied Phycology. 33:2957-2966 |
| ISSN: | 1573-5176 0921-8971 |
| Popis: | Astaxanthin is attracting considerable interest due to its high inhibitory power of oxidative stress in humans and can be produced by the microalga Haematococcus pluvialis. However, the highly physicochemical resistant cell wall of H. pluvialis poses an important challenge in extracting intracellular compounds. We therefore conducted a multiple variable evaluation of acid hydrolysis for cell disruption of wet H. pluvialis biomass, followed by solid–liquid extraction with GRAS (generally recognized as safe) solvents. Preliminary tests selected hydrochloric acid as the most suitable acid for hydrolysis and acetone as the most appropriate solvent for solid–liquid extraction. A central composite design and response surface model were utilized to predict the best conditions for astaxanthin recovery. The variables evaluated were acid concentration (0.5–4.5 N), hydrolysis time (3.2–16.8 min), and temperature (56–84 °C). The highest astaxanthin recovery (99 ± 0.48%) was achieved with the following condition: temperature (T) of 71 °C, time (t) of 17 min, and [HCl] = 3.7 N. The model indicates that high astaxanthin recovery (97%) can be achieved while reducing [HCl] to 2.3 N, positively impacting the cost and safety of this method. Despite the high solubility of astaxanthin in non-polar solvents, the wet cells of H. pluvialis favored the diffusion of solvents with higher water miscibility. Acid hydrolysis, even under the optimal conditions, did not cause total cell wall rupture, indicating that solvent accessibility occurred through microperforations in the cell wall. Overall, the results establish a more profound understanding on conditions for efficient processing of wet H. pluvialis biomass directly from harvesting operation. |
| Databáze: | OpenAIRE |
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