Oligonucleotide conjugates based on acyclonucleosides and their use in DNA hybridization assays

Autor:	Harri Hakala, Jenni Degerholm, Pia Ollikka, Jari Hovinen
Rok vydání:	2002
Předmět:	Lanthanide DNA synthesis DNA-encoded chemical library Oligonucleotide Chemistry DNA–DNA hybridization Organic Chemistry Drug Discovery Chelation Biochemistry Combinatorial chemistry Conjugate
Zdroj:	Tetrahedron. 58:8771-8777
ISSN:	0040-4020
DOI:	10.1016/s0040-4020(02)01056-6
Popis:	Synthesis of two oligonucleotide building blocks based on acyclonucleosides (10, 11) which enable the introduction of several nonluminescent and luminescent lanthanide(III) chelates to the oligonucleotide structure is described. They were used in an instrument-assisted DNA synthesis in a standard manner. A modified deprotection procedure was used to ensure metal complexation. Also the applicability of these oligonucleotide conjugates to DNA hybridization assays is demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::57cad3ed4ac11e241abae277a296feba https://doi.org/10.1016/s0040-4020(02)01056-6 Zobrazit plný text záznamu Full Text from ScienceDirect