| Autor: | David I. Stirling, Mei He, Mark L Kayton, Elise C. Kohn, Sarah O'Connor, Vladimir Knezevic, George W. Muller, Walter J. Miller, Galina Baibakov, Angela M. Patton, Huan Vu, Dominique Lorang, Steven K. Libutti, H. Richard Alexander, Faribourz Payvandi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Drug discovery General Medicine Biology General Biochemistry Genetics and Molecular Biology Protein expression Chick chorioallantoic membrane Cell biology law.invention Endothelial stem cell In vivo Confocal microscopy law medicine sense organs skin and connective tissue diseases Cam assay |
| Zdroj: | Journal of Translational Medicine. 2:4 |
| ISSN: | 1479-5876 |
| DOI: | 10.1186/1479-5876-2-4 |
| Popis: | Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response. |
| Databáze: | OpenAIRE |
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