Immobilization ofMucor javanicuslipase by entrapping in alginate-silica hybrid gel beads with simultaneous cross-linking with glutaraldehyde

Autor:	Yvonne Chow, Chor Yong Tay, Yew Kong Wong, Mmr Talukder, Jin Chuan Wu, Won Jae Choi, Kiat Wei Chang
Rok vydání:	2007
Předmět:	Carboxylic Ester Hydrolases Chromatography biology technology industry and agriculture Triacylglycerol lipase macromolecular substances biology.organism_classification Biochemistry Catalysis Enzyme assay stomatognathic diseases chemistry.chemical_compound chemistry Mucor circinelloides biology.protein Mucor javanicus Glutaraldehyde Lipase Biotechnology
Zdroj:	Biocatalysis and Biotransformation. 25:459-463
ISSN:	1029-2446 1024-2422
DOI:	10.1080/10242420701661149
Popis:	Mucor javanicus lipase was entrapped in alginate-silica hybrid gel beads with or without simultaneous cross-linking with glutaraldehyde. The activity and recovery of activity on immobilization of the enzyme entrapped in the hybrid beads were 1.4 and 1.7 times higher than those of the enzyme entrapped in the simple alginate beads. Entrapment with simultaneous cross-linking in the hybrid beads further improved the enzyme activity (1.6 times) and activity recovery (1.7 times) compared to those of the enzyme entrapped in the hybrid beads without simultaneous cross-linking. The leakage of the enzyme entrapped in the hybrid beads with simultaneous cross-linking was only 50% that of the enzyme entrapped in the simple alginate beads.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::573c21675b0ff9c60bb2f9741dd74126 https://doi.org/10.1080/10242420701661149 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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