| Autor: |
Sam Daly, João Ferreira Fernandes, Ezra Bruggeman, Anoushka Handa, Ruby Peters, Sarah Benaissa, Boya Zhang, Joseph S. Beckwith, Edward W. Sanders, Ruth R. Sims, David Klenerman, Simon J. Davis, Kevin O’Holleran, Steven F. Lee |
| Rok vydání: |
2023 |
| DOI: |
10.1101/2023.05.02.539032 |
| Popis: |
Volumetric super-resolution microscopy typically encodes the 3D position of single-molecule fluorescence into a 2D image by changing the shape of the point spread function (PSF) as a function of depth. However, the resulting large and complex PSF spatial footprints reduce temporal resolution by requiring lower labelling densities to avoid overlapping fluorescent signals. We quantitatively compare the density dependence of single-molecule light field microscopy (SMLFM) to other 3D PSFs (astigmatism, double helix and tetrapod) showing that SMFLM enables an order-of-magnitude speed improvement compared to the double helix PSF by resolving overlapping emitters through parallax. We then experimentally demonstrate the high accuracy (>99.2 ± 0.1%, 0.1 locs μm−2) and sensitivity (>86.6 ± 0.9%, 0.1 locs μm−2) of SMLFM at point detection through whole-cell (scan-free) imaging and tracking of single membrane proteins in live primary B cells. We also exemplify high density volumetric imaging (0.15 locs μm−2) in dense cytosolic tubulin datasets. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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