Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

Autor:	Ana Uclés, Antonio Valverde García, Ana Aguilera-del Real, Amadeo R. Fernández-Alba, María Dolores Gil García
Rok vydání:	2015
Předmět:	Wine Detection limit Benzimidazole Chromatography Pesticide residue Carbendazim General Chemical Engineering General Engineering food and beverages Analytical Chemistry Triple quadrupole mass spectrometer Fungicide chemistry.chemical_compound chemistry Imidazole
Zdroj:	Analytical Methods. 7:9158-9165
ISSN:	1759-9679 1759-9660
Popis:	This manuscript reports a quantitative pesticide residue method analysis employing an enzyme-linked immunosorbent assay (ELISA) for detecting carbendazim, imazalil and thiabendazole pesticide residues in vegetable samples. The results have been compared with those obtained from a liquid chromatography system coupled to a triple quadrupole mass spectrometer (LC-ESI-QqQ-MS). A validation study was undertaken to establish this method's performance characteristics on the 3 pesticide residues in two commodities (table grapes and wine). The carbendazim, imazalil and thiabendazole ELISA showed an IC50 reporting limit ranging from 0.5 to 13.8 µg kg−1. The limit of quantification of the developed assay was 5 µg kg−1 for both grape and wine matrices. The optimised assay was compared with a reference procedure based on liquid chromatography using samples acquired from various supermarkets, and a good correlation between both methods was found.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::552fd68e9c83daa9512e8bd761f29943 https://doi.org/10.1039/c5ay01048a Zobrazit plný text záznamu