OMIP-048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
| Autor: | Agnieszka Jaracz-Ros, Patrice Hémon, Hélène Gary-Gouy, Géraldine Schlecht-Louf, Françoise Bachelerie, Roman Krzysiek |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Histology biology chemistry.chemical_element STIM1 Cell Biology Calcium Outer mitochondrial membrane Pathology and Forensic Medicine Cell biology Cell membrane 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry medicine biology.protein Mass cytometry Antibody VDAC1 030215 immunology Lymphocyte subsets |
| Zdroj: | Cytometry Part A. 93:681-684 |
| ISSN: | 1552-4922 |
| DOI: | 10.1002/cyto.a.23504 |
| Popis: | Calcium (Ca2+ ) signaling controls T-cell activation and functions. Ca2+ concentrations are locally detected and controlled by Ca2+ -sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca2+ -channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca2+ -sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets. |
| Databáze: | OpenAIRE |
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