| Popis: |
Rationale: Metabolome represents the collection of low molecular weight compounds that originate from metabolic pathways of the body, drugs, and microorganisms in the oral cavity. Objectives: The aim of study was to evaluate the biofluid metabolome of kidney transplant recipients and their possible association with periodontitis. Materials and Methods: This was a cross-sectional, analytical, and descriptive study involving 61 patients, who visited the Dental School of Paulist University. The patients were arranged in four groups: GT: kidney transplant recipients without periodontal disease (n = 11); GTDP: kidney transplant recipients with periodontal disease (n = 12); CG: normal renal function without periodontal disease (n = 19); GCDP: normal renal function with periodontal disease (n = 19). Saliva and urine samples were collected for further metabolomic analysis. Statistical analyses were performed using the principal component analysis (PCA), Venn diagram, Jaccard index, and Permanova. Results: The study identified 23 and 30 metabolites common to the GT and GTDP groups when the saliva and urine samples were analyzed, respectively; the benzene class and its derivatives were overexpressed. Metabolites expressed in groups with periodontal disease were related to bacterial metabolism and anaerobic cell respiration. The most important metabolite for determining the pattern of PCA performed in saliva and urine samples was ornithine and palmitoleic acid, respectively. Conclusions: There was no significant difference between the salivary and urinary metabolites when compared among the four groups; however, it was possible to differentiate the specific metabolites for each group and the relationships between them. Metabolites shared in salivary samples of patients with periodontal disease are related to fermentation process and bacterial metabolism, suggesting that they are potential indicators for periodontal injury. |
