Effect of Salinity Stress on Glutathione –S-Transferases (GSTs) of Maize

Autor: N. A. Ivy, M. Rohman, R. N. Remme, M. A. K. Mian
Rok vydání: 2013
Předmět:
Zdroj: IOSR Journal of Agriculture and Veterinary Science. 4:42-52
ISSN: 2319-2380
2319-2372
Popis: The present study was undertaken to explore the responses of maize GST to salinity. GST activities were found to induce with the increasing of salinity levels and duration of stress. At 3 day, 5 day and 7 day stress most of all the salinity concentration (4 dS/m, 8 dS/m and 12 dS/m) increased the GST activities over control. However 16 dS/m salinity decreased the activity over control. In foliar application of proline GST activities were increased gradually over control and NaCl treatment at 3 day, 5 day and 7 day stress in all the salinity level. In betaine application at 4 dS/m, 8 dS/m, 12 dS/m and 16 dS/m salinity also increased GST activity were gradually over control at 3 day, 5 day and 7 day stress. It also increases the GST activity during the stress period over NaCl stress in all the salinity levels, except 12 dS/m and 16 dS/m salinity at 3 day stress. When both proline and betaine applied together in stressed seedlings at 4 dS/m, 8 dS/m, 12 dS/m and 16 dS/m salinity increased the GST activity at 3 day, 5 day and 7 day stress over control. Though the combination of 15 mM proline and betaine decreased the activities at 3 day and 7 day stress at 4 dS/m, 8 dS/m and 12 dS/m at 3 day stress GST activity increased at other salinity stress in different stress period. To examine the detoxification role of maize GST, concentration of MDA (peroxident product of poly unsaturated fatty acid) in the stressed maize seedlings was studied. In all the salinity levels, MDA contents increased over control. Supplementation of proline and betaine reduced the concentrations of MDA over salinity stresses. The simultaneous increased GST activities and lower MDA concentration suggested the detoxification role of maize GST on cytotoxic MDA.
Databáze: OpenAIRE
Externí odkaz: