In vivodiffuse reflectance micro-spectroscopy for correction of Raman depth profiles acquired on skin
| Autor: | Michel Manfait, Francois Perraut, Blandine Roig, Jean-Marc Dinten, Olivier Piot, A. Koenig |
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| Rok vydání: | 2016 |
| Předmět: |
Elastic scattering
Materials science Diffuse reflectance infrared fourier transform Scattering business.industry Attenuation Physics::Medical Physics 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 010309 optics symbols.namesake Optics Attenuation coefficient 0103 physical sciences symbols Diffuse reflection 0210 nano-technology Spectroscopy business Raman spectroscopy |
| Zdroj: | Biophotonics: Photonic Solutions for Better Health Care V. |
| ISSN: | 0277-786X |
| Popis: | Confocal Raman microspectroscopy is a relevant and useful tool to perform in vivo diagnosis of cutaneous tissues noninvasively and without labeling. This optical technique provides in-depth molecular and conformational characterization of skin. Unfortunately, spectral distortions occur due to elastic scattering. Our objective is to correct the attenuation of in-depth Raman peaks intensity by considering elastic scattering in biological tissues. In this purpose, a correction model was constructed using skin scattering properties as parameters thus enabling quantitative analysis. The work presented here is a technique of in vivo Diffuse Reflectance Micro-Spectroscopy called Micro-DRS. It achieves optical properties characterization in the skin layers probed by Raman microspectroscopy. The Micro-DRS setup can easily be coupled to a confocal Raman micro-probe to perform simultaneous measurements. Thanks to Monte Carlo simulations and experimental results obtained on homemade solid phantoms mimicking skin optical properties, we show that it is possible to measure the absorption coefficient μ a , the reduced scattering coefficient μ s ', the scattering coefficient μ s and the anisotropy of scattering g with this new apparatus. The measured scattering properties can be used subsequently as parameters in our correction model. Coupled to a Raman micro-spectrometer, Micro-DRS enables a quantitative analysis when tracking drug penetration through skin and it can be used independently to provide additional diagnosing criterions. |
| Databáze: | OpenAIRE |
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