Agro-industrial-Produced Laccase for Degradation of Diclofenac and Identification of Transformation Products
| Autor: | Leticia Hernandez Galan, Rao Y. Surampalli, Mausam P. Verma, Linson Lonappan, Tarek Rouissi, Satinder Kaur Brar, Mohamed Amine Laadila |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
General Chemical Engineering 010501 environmental sciences engineering.material 01 natural sciences Mineralization (biology) Ammonia chemistry.chemical_compound 010608 biotechnology Environmental Chemistry Food science 0105 earth and related environmental sciences 2. Zero hunger Laccase Phenol red Renewable Energy Sustainability and the Environment business.industry Chemistry Pulp (paper) Pomace Substrate (chemistry) General Chemistry Biotechnology engineering Degradation (geology) business |
| Zdroj: | ACS Sustainable Chemistry & Engineering. 5:5772-5781 |
| ISSN: | 2168-0485 |
| DOI: | 10.1021/acssuschemeng.7b00390 |
| Popis: | A widely used anti-inflammatory drug, diclofenac (DCF), is recalcitrant in many environmental compartments and poses threat to several aquatic and terrestrial organisms. Enzymatic degradation of emerging contaminants which are often micropollutants, has gained interest for the past few years. However, production of enzymes often incurs high costs. In this study, ligninolytic enzyme laccase was produced by white rot fungi Tremetes versicolor (ATCC 20869) using agro-industrial residues, apple pomace (AP), pulp and paper solid waste (PPSW), and alfa fibers as substrates. Various known inducers for laccase production, such as tween 80 (0.1% (w/w)), veratryl alcohol (3 mM Kg–1), CuSO4 (3 mM Kg–1), and phenol red (3 mM Kg–1) were used to enhance laccase production. A maximum laccase activity of 49.16 ± 4.5, 52.4 ± 2.2, and 14.26 ± 0.8 U/gds (units/gram dry substrate) was obtained from apple pomace, PPSW, and alfa plant fibers, respectively, at optimal experimental conditions. Further, the kinetics of the laccas... |
| Databáze: | OpenAIRE |
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