Iron–Sulfur Clusters in Zinc Finger Proteins
| Autor: | Geoffrey D. Shimberg, Jordan D. Pritts, Sarah L. J. Michel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Zinc finger biology Iron–sulfur cluster chemistry.chemical_element Zinc Cleavage and polyadenylation specificity factor Cofactor 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry biology.protein Peptide sequence Histidine Cysteine |
| DOI: | 10.1016/bs.mie.2017.09.005 |
| Popis: | Zinc finger (ZF) proteins are proteins that use zinc as a structural cofactor. The common feature among all ZFs is that they contain repeats of four cysteine and/or histidine residues within their primary amino acid sequence. With the explosion of genome sequencing in the early 2000s, a large number of proteins were annotated as ZFs based solely upon amino acid sequence. As these proteins began to be characterized experimentally, it was discovered that some of these proteins contain iron-sulfur sites either in place of or in addition to zinc. Here, we describe methods to isolate and characterize one such ZF protein, cleavage and polyadenylation specificity factor 30 (CPSF3O) with respect to its metal-loading and RNA-binding activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|