Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate

Autor:	J F Wedgwood, J L Strominger
Rok vydání:	1980
Předmět:	chemistry.chemical_classification Phosphatase Cell Biology Bacitracin Dolichyl pyrophosphate Biochemistry Chemical synthesis Pyrophosphate chemistry.chemical_compound Enzyme Membrane chemistry medicine Methylene Molecular Biology medicine.drug
Zdroj:	Journal of Biological Chemistry. 255:1120-1123
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)86150-1
Popis:	Enzymatic activities which dephosphorylate dolichyl phosphate (Dol-P) and dolichyl pyrophosphate (Dol-P-P) have been observed in membranes from cultured human lymphocytes. Neither activity requires divalent metals. Dol-P phosphatase is inhibited by inorganic phosphate but not by other phosphate-containing compounds. Dol-P-P phosphatase is inhibited by bacitracin but not by phosphate-containing compounds including the methylene analogue of pyrophosphate. These reactions are similar to those previously found in the cycle of bacterial wall peptidoglycan biosynthesis. A chemical synthesis of [32P]Dol-P and [32P]Dol-P-P is reported.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::509d5ed3285568926c4ad73714a9f37c https://doi.org/10.1016/s0021-9258(19)86150-1 Zobrazit plný text záznamu