Rat insulin-like growth factor-I and -II mRNAs are unchanged during compensatory adrenal growth but decrease during ACTH-induced adrenal growth
| Autor: | S F Townsend, Walter L. Miller, M F Dallman |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
endocrine system
medicine.medical_specialty Adrenalectomy medicine.medical_treatment Growth factor Cell Biology Adrenocorticotropic hormone Peptide hormone Biology Biochemistry Somatomedin Insulin-like growth factor Endocrinology Internal medicine Insulin-like growth factor 2 medicine biology.protein Autocrine signalling Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 265:22117-22122 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(18)45678-5 |
| Popis: | The insulin-like growth factors (IGFs) may be important autocrine and paracrine mediators of organ growth. We used solution-hybridization/ribonuclease protection assays to examine IGF-I and IGF-II mRNA abundance during hypertrophy or the rat adrenal gland induced by unilateral adrenalectomy or by adrenocorticotropic hormone (ACTH) infusion. Adrenal IGF-I mRNA did not change during the period of rapid organ growth at 18 or 66 h after unilateral adrenalectomy. ACTH infusion induced a time- and dose-dependent decrease in adrenal IGF-I mRNA despite significant increases in gland size. IGF-II mRNA also remained unchanged after unilateral adrenalectomy and decreased after ACTH infusion, to a greater extent than IGF-I mRNA. Liver IGF-I mRNA did not change with ACTH exposure, indicating an effect specific to the adrenal. We also measured adrenal P450scc mRNA as a marker of steroidogenic capacity. P450scc mRNA was unchanged after unilateral adrenalectomy and increased with ACTH infusion. Thus IGF-I and IGF-II mRNAs respond in parallel, but in different fashions with different stimuli for adrenal growth. The decrease in IGF mRNA after exposure to ACTH may be a factor in the ACTH-induced inhibition of compensatory hypertrophy after unilateral adrenalectomy. |
| Databáze: | OpenAIRE |
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