Determination of clenbuterol in urine by azo-dye precolumn derivatization and micellar liquid chromatography
| Autor: | J. J. Baeza Baeza, Guillermo Ramis-Ramos, Y.Martin Biosca |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Detection limit
Chromatography Chemistry Elution Organic Chemistry Clinical Biochemistry Extraction (chemistry) Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Micellar liquid chromatography Solid phase extraction Derivatization |
| Zdroj: | Chromatographia. 44:145-150 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02466448 |
| Popis: | Clenbuterol has been determined in urine by solidphase extraction on a C18 cartridge, diazotization of the eluate with nitrite, coupling of the diazonium ion with 1-(naphthyl)ethylenediamine, and separation of the azo dye formed by HPLC with a C18 column and a micellar mobile phase containing 0.1 M sodium dodecyl sulphate, 12%n-butanol and 0.05 M citrate buffer, pH 3. Recoveries higher than 90% were obtained by mixing the samples with a 20% 0.2 M NaOH before extraction. Limits of detection of 51 and 6.7 ng L−1 were obtained with spectrophotometric and thermal lens spectrometric detection, respectively; respective repeatabilities were 3.1% (5 μg mL−1) and 5.6% (0.16 μg mL−1). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink